April 23, 2006, ACD/Labs ENC 2006 Academia Seminar, Pacific Grove, CA, USA

HR-MAS Spectroscopy of Prostate Cancer Tissues

Mark G. Swanson

Abstract

High resolution magic angle spinning (HR-MAS) spectroscopy is an emerging technique for the metabolic characterization of intact tissues, cells, and biofluids. However, these studies are challenging because the data must be acquired as fast and as efficiently as possible to preserve the metabolic, pathologic, and RNA integrity of the specimens for subsequent analyses. A confounding problem is that conventional 1D HR-MAS spectra typically suffer from severe spectral overlap such that 2D NMR and/or spectral editing approaches are necessary to extract the metabolic information. In this presentation, we describe quantitative HR-MAS analysis of prostate metabolites, strategies for selective excitation spectral editing, and 2D spectroscopy (TOCSY) techniques using rotor synchronized adiabatic pulses. Current HR-MAS studies involving prostate biopsy and surgical tissues combined with pathologic and genetic microarray analysis will also be described.

Download the presentation in MS PowerPoint (7.52 Mb ZIP file) or Adobe Acrobat format (827 Kb PDF file).

Relevant Product: ACD/1D NMR Processor

Relevant Solution: Metabolism Studies, Tools for Teaching Chemistry