HPLC 2008

May 10–16, 2008

Marriott Waterfront Hotel
Baltimore, MD, USA

Conference Details

Booth #510

Poster Schedule

Poster Presentation: May 13–15, 2008

TITLE: LC/MS/UV Data Reduction in Purity and Stability-Indicating HPLC Method Development
Rudy Sneyers and Willy Janssens (Johnson and Johnson) and Mike McBrien (ACD/Labs)

Abstract: The systematic parameter investigation and hyphenated (LC/UV and LC/MS) data collection associated with chromatographic method development tend to result in considerable data overload. Increased rigour of development inherently implies more data, and thus even more data processing requirements. Nowhere is this phenomenon more evident than in the situation of development of chromatographic methods for stability measurements. In these situations, it is typical to have several individual samples that represent the overall elution profile of the expected compounds associated with the project. The amount of data collected increases proportionally to the number of samples, which can result in a vast array of files that must be fully processed before experimental design can be effective. This paper will detail an approach to the effective extraction of key elution information for compounds associated with degradation of pharmaceutical compounds based on targeted selection of samples to include in the study, scientific design of appropriate method design space, chemometric data reduction, and other novel data reduction approaches. The result is fast availability of key elution data to method design tools. The approach will be illustrated with several real-world examples.

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